Characterization and functional interrogation of the SARS-CoV-2 RNA interactome
In: ISSN: 2211-1247, 2022
Online
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Zugriff:
International audience ; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutral- izing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valu- able resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies.
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Characterization and functional interrogation of the SARS-CoV-2 RNA interactome
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Autor/in / Beteiligte Person: | Labeau, Athéna ; Fery-Simonian, Luc ; Lefevre-Utile, Alain ; Pourcelot, Marie ; Bonnet-Madin, Lucie ; Soumelis, Vassili ; Lotteau, Vincent ; Vidalain, Pierre-Olivier ; Amara, Ali ; Meertens, Laurent ; Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH) (IRSL) ; Université Paris Cité (UPCité) ; Centre International de Recherche en Infectiologie (CIRI) ; École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) ; This work was supported by the French government's Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (grant no. ANR-10-LABX-62-IBEID), the Fondation pour la Recherche Médicale (grant no. FRM-EQU202003010193), the Agence Nationale de la Recherche (ANR/FRM Flash COVID project IDISCOVR and ANR-20-CO11-0004 project FISHBP), and University Paris Cité (Plan de Soutien Covid-19: RACPL20FIR01-COVID-SOUL). The authors thank Alessia Zamborlini for critical reading of the manuscript. L.M. and A.A. dedicate this work to the memory of Professor Jean-Louis Virelizier (Unité d’Immunologie Virale, Institut Pasteur, Paris) and Professor Renaud Mahieux (Ecole Normale Supérieure, Lyon, France), who left us during the SARS-CoV-2 epidemic. ; ANR-20-CO11-0004,FISHBP,Compréhension de la fonction des facteurs cellulaires interagissant avec l'ARN de SARS-COV-2 durant la réplication virale(2020) ; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) |
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Zeitschrift: | ISSN: 2211-1247, 2022 |
Veröffentlichung: | HAL CCSD ; Elsevier Inc, 2022 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.celrep.2022.110744 |
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