Delayed lgG2 humoral response in infants is not due to intrinsic T or B cell defects
Oxford University Press, 1996
academicJournal
Zugriff:
The physiologically low or absent lgG2 responses of infants have been attributed to T or B cell functional immaturity. We have analyzed the capacity of adult and neonatal T lymphocytes to secrete lgG2 switch factor (lgG2-SF) and the capacity of neonatal B cells to respond to such factors. The lgG2-SF capacity was assessed on CD40-activated naive B cells, measuring lgG2 by ELISA in supernatants of cultures performed in the presence of IL-10. T cells secreted lgG2-SF together with IL-2 and IFN-γ, after activation with a combination of anti-CD2, anti-CD28 and phorbol myristate acetate (T h 1-like activation). In contrast, activation with anti-CD3 and anti-CD28, which yielded IL-4 and IL-10 but neither IL-2 nor IFN-γ(T h 2-like activation), did not result in the secretion of lgG2-SF. The supernatant of activated neonatal T cells contained lgG2-SF. Neonates' B cells produced almost as much lgG2 as did naive adult B cells. The effect of lgG2-SF was further demonstrated by its ability to induce 3–15% of CD40-activated naive B cells to express cytoplasmic lgG2 regardless of the presence of IL-10. This study demonstrates that: (i) lgG2 switch can be T cell dependent in humans, (ii) lgG2-SF is produced with T h 1-like cytokines and (iii) low lgG2 responses in infants do not result from either an inability of T cells to produce lgG2-SF or an inability of B cells to undergo lgG2 switch in vitro .
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Delayed lgG2 humoral response in infants is not due to intrinsic T or B cell defects
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Autor/in / Beteiligte Person: | Servet-Delprat, Christine ; Bridon, Jean-Michel ; Djossou, Odile ; Yahia, Smina Ait ; Banchereau, Jacques ; Brière, Francine |
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Veröffentlichung: | Oxford University Press, 1996 |
Medientyp: | academicJournal |
DOI: | 10.1093/intimm/8.10.1495 |
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