Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota
In: ISSN: 1932-6203, 2019
Online
academicJournal
Zugriff:
International audience ; The honeybee (Apis mellifera) has to cope with multiple environmental stressors, especially pesticides. Among those, the herbicide glyphosate and its main metabolite, the amino-methylphosphonic acid (AMPA), are among the most abundant and ubiquitous contaminant in the environment. Through the foraging and storing of contaminated resources, honey-bees are exposed to these xenobiotics. As ingested glyphosate and AMPA are directly in contact with the honeybee gut microbiota, we used quantitative PCR to test whether they could induce significant changes in the relative abundance of the major gut bacterial taxa. Glyphosate induced a strong decrease in Snodgrassella alvi, a partial decrease of a Gillia-mella apicola and an increase in Lactobacillus spp. abundances. In vitro, glyphosate reduced the growth of S. alvi and G. apicola but not Lactobacillus kunkeei. Although being no bee killer, we confirmed that glyphosate can have sublethal effects on the honeybee microbiota. To test whether such imbalanced microbiota could favor pathogen development, honeybees were exposed to glyphosate and to spores of the intestinal parasite Nosema ceranae. Glyphosate did not significantly enhance the effect of the parasite infection. Concerning AMPA, while it could reduce the growth of G. apicola in vitro, it did not induce any significant change in the honeybee microbiota, suggesting that glyphosate is the active component modifying the gut communities.
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Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota
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Autor/in / Beteiligte Person: | Blotid, Nicolas ; Veillat, Loïs ; Rouzé, Régis ; Delatte, Hélène ; Laboratoire Microorganismes : Génome et Environnement (LMGE) ; Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Centre National de la Recherche Scientifique (CNRS) ; Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
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Zeitschrift: | ISSN: 1932-6203, 2019 |
Veröffentlichung: | HAL CCSD ; Public Library of Science, 2019 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0215466 |
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