Printed in Great Britain © Company of Biologists Limited IQJ8 MACROTUBULES INDUCED BY HALOTHANE: IN VITRO ASSEMBLY
In: http://jcs.biologists.org/content/32/1/99.full.pdf
Online
academicJournal
Zugriff:
The formation of macrotubules by the volatile anaesthetic halothane was investigated in vitro using mjcrotubule-enriched fractions of crayfish nerve cords. Sequential studies showed that macrotubules assemble from helical ribbons of 18—20 laterally associated micro tubule proto-filaments which fold upon themselves to form intact macrotubules averaging 48 run in diameter. The initial rate of macrotubule assembly is dependent on the concentration of halothane employed and is stimulated by calcium. Glycerol pretreatment blocked macrotubule formation by halothane and caused preformed macrotubules to reassemble rapidly into typical micro-tubules. These experiments show that microtubules and macrotubules require different con-ditions for assembly and support the contention that macrotubule formation by halothane is due to a direct interaction between the anaesthetic molecule and the microtubule subunit.
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Printed in Great Britain © Company of Biologists Limited IQJ8 MACROTUBULES INDUCED BY HALOTHANE: IN VITRO ASSEMBLY
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Autor/in / Beteiligte Person: | Hinkley, Robert E. ; The Pennsylvania State University CiteSeerX Archives |
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Zeitschrift: | http://jcs.biologists.org/content/32/1/99.full.pdf |
Medientyp: | academicJournal |
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