TTV基因酶切分型研究 ; Typing of TTV genomes by restriction fragment length polymorphism
In: 万方 ; http://d.g.wanfangdata.com.cn/Periodical_lcjyzz200101003.aspx, 2001
Online
academicJournal
Zugriff:
目的了解中国TTV基因分型状况。方法采用套式PCR检测D8、D10、D22、D25、D29血清中TTVDNA，限制性内切酶和PCR产物直接测序技术进行酶切和序列分析。结果N22～D8存在BstUI特异性切点，G1b～D10无BstUI切点，有EcoRI切点，G2a、D25、D29序列均存在HaeⅢ和Tsp509切点，G2b～D22除存在HaeⅢ切点之外还具有MboⅠ切点。Pat3～TTh6仅有HaeⅢ切点。结论AB008394～D8具有相同的切点可能均属1a型，G1b～Jcase5切点相同可能为1b型，TTh1～1和TTh1～2可能属1型中的其它亚型。D25～D29酶切点相同为2a型，G2b～D22切点相同为2b型，Pat3～TTh6切点相同可能为2型中的其它亚型。 ; Objectives To study the typing of TTV genomes by restriction fragment length polymorphisms(RFLPs).Methods TTV DNA was detected by PCR products of reverse transcriptase-nested PCR in D8,D10,D22,D25,D29 sera.The PCR products of restriction endoclease digestion and that of direct sequence analysis were used in the typing of TTV genomes.Results N22-D8 isolates had Bst U I site,and G1b-D10 isolates without Bst U I site had EcoR I site,G2a、 D25、 D29 isolates had both Hae Ⅲ and Tsp509 site.G2b-D22 had Mbo Ⅰ site beside Hae Ⅲ site .Pat3-TTh6 isolates only had Hae Ⅲ site .Conclusion AB008394-D8 isolates with the identical site belonged to type-la.G1b-Jcase isolates with the identical site belonged to type-1b.And TThl-2 belonged to other subtypes of type-1,D25-D29 isolates belonged to type-2a,and G2b-D22 isolates to type-2b.However,Pat3-TTh6 isolates were thought as members of subtypes type-2. ; 中文核心期刊要目总览(PKU) ; 中国科学引文数据库(CSCD) ; 0 ; 1 ; 8-10 ; 19
| Titel: |
TTV基因酶切分型研究 ; Typing of TTV genomes by restriction fragment length polymorphism
|
|---|---|
| Autor/in / Beteiligte Person: | 杜绍财 ; 朱凌 ; 季颖 ; 张敏 ; 张玲荣 ; 北京大学人民医院肝病研究所 ; 山西医科大学第一附属医院传染科 |
| Link: | |
| Zeitschrift: | 万方 ; http://d.g.wanfangdata.com.cn/Periodical_lcjyzz200101003.aspx, 2001 |
| Veröffentlichung: | 临床检验杂志, 2001 |
| Medientyp: | academicJournal |
| ISSN: | 1001-764X (print) |
| DOI: | 10.3969/j.issn.1001-764X.2001.01.003 |
| Schlagwort: |
|
| Sonstiges: |
|