Recombinant Fc of human lgG1 prepared in an Escherichia coli system escapes recognition by macrophages
Oxford University Press, 1990
academicJournal
Zugriff:
The Fc portion of IgG is recognized by macrophages via Fc receptors on their cell surface. The recognition of human lgG1-Fc by macrophages was compared for the native and recomblnant proteins by a novel assay method using competitive inhibition of the aggregated IgG-medlated chemllumlnescence reaction of human macrophage cell line THP-1. Recombinant Fc proteins, prepared in an Escherichia coll system, which lack ollgosaccharides and retain normal staphylococcal protein A reactivity and, to a lesser extent, C1q binding capacity, showed Inhibitory ability >500 times lower than that of native Fc fragments obtained by proteolytlc digestion of myeloma proteins. This suggests that the oligosaccharlde chains on lgG1-Fc molecules may be critical for recognition by Fc receptors on macrophages.
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Recombinant Fc of human lgG1 prepared in an Escherichia coli system escapes recognition by macrophages
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Autor/in / Beteiligte Person: | Nose, Masato ; Takano, Ryo ; Nakamura, Satoshi ; Arata, Yoji ; Kyogoku, Masahisa |
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Veröffentlichung: | Oxford University Press, 1990 |
Medientyp: | academicJournal |
DOI: | 10.1093/intimm/2.11.1109 |
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