Exploratory pilot study to characterize the immune landscapes of malignant pleural effusions and their corresponding primary tumors from patients with breast carcinoma and lung adenocarcinoma.

Laberiano-Fernandez, C ; Gan, Q ; et al.

In: Journal of the American Society of Cytopathology, Jg. 13 (2024-05-01), Heft 3, S. 161-173

academicJournal

Introduction: Malignant pleural effusion (MPE) is a frequent complication of advanced malignancies. In this pilot study, we characterized the immune landscapes of MPEs, compared them to their primary tumor (PT) samples from breast carcinoma (BC) and lung adenocarcinoma (LADC), and tested the utility of multiplexed image technology in cytological samples.

Materials and Methods: We evaluated the immune contexture of 6 BC and 5 LADC MPEs and their PTs using 3 multiplex immunofluorescence panels. We explored the associations between sample characteristics and pleural effusion-free survival.

Results: No MPE samples had positive programmed death-ligand 1 expression in malignant cells, although 3 of 11 PTs has positive programmed death-ligand 1 expression (more than 1% expression in malignant cells). Overall, in LADC samples, cluster of differentiation 3 (CD3)+ T cells and CD3+CD8+ cytotoxic T cells predominated (median percentages for MPEs versus PTs: 45.6% versus 40.7% and 4.7% versus 6.6%, respectively) compared with BC. CD68+ macrophages predominated in the BC samples (medians for MPEs 61.2% versus PTs for 57.1%) but not in the LADC samples. Generally in PTs, CD3+CD8+ forkhead box P3+ T cells and the median distances from the malignant cells to CD3+CD8+Ki67+ and CD3+ programmed cell death protein 1 + T cells correlated to earlier MPE after PT diagnosis.

Conclusions: The immune cell phenotypes in the MPEs and PTs were similar within each cancer type but different between BC versus LADC. An MPE analysis can potentially be used as a substitute for a PT analysis, but an expanded study of this topic is essential.

Titel:	Exploratory pilot study to characterize the immune landscapes of malignant pleural effusions and their corresponding primary tumors from patients with breast carcinoma and lung adenocarcinoma.
Autor/in / Beteiligte Person:	Laberiano-Fernandez, C ; Gan, Q ; Wang, SM ; Tamegnon, A ; Wistuba, I ; Yoon, E ; Roy-Chowdhuri, S ; Parra, ER
Zeitschrift:	Journal of the American Society of Cytopathology, Jg. 13 (2024-05-01), Heft 3, S. 161-173
Veröffentlichung:	New York, NY : Elsevier, 2024
Medientyp:	academicJournal
ISSN:	2213-2945 (print)
DOI:	10.1016/j.jasc.2024.02.005
Schlagwort:	Humans Female Pilot Projects Middle Aged Aged B7-H1 Antigen immunology B7-H1 Antigen metabolism Male Adenocarcinoma pathology Adenocarcinoma immunology Adenocarcinoma diagnosis Adult Aged, 80 and over Biomarkers, Tumor immunology Breast Neoplasms immunology Breast Neoplasms pathology Breast Neoplasms diagnosis Pleural Effusion, Malignant immunology Pleural Effusion, Malignant pathology Lung Neoplasms pathology Lung Neoplasms immunology Adenocarcinoma of Lung pathology Adenocarcinoma of Lung immunology Adenocarcinoma of Lung diagnosis
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Am Soc Cytopathol] 2024 May-Jun; Vol. 13 (3), pp. 161-173. <i>Date of Electronic Publication: </i>2024 Feb 29. MeSH Terms: Breast Neoplasms* / immunology ; Breast Neoplasms* / pathology ; Breast Neoplasms* / diagnosis ; Pleural Effusion, Malignant* / immunology ; Pleural Effusion, Malignant* / pathology ; Lung Neoplasms* / pathology ; Lung Neoplasms* / immunology ; Adenocarcinoma of Lung* / pathology ; Adenocarcinoma of Lung* / immunology ; Adenocarcinoma of Lung* / diagnosis ; Humans ; Female ; Pilot Projects ; Middle Aged ; Aged ; B7-H1 Antigen / immunology ; B7-H1 Antigen / metabolism ; Male ; Adenocarcinoma / pathology ; Adenocarcinoma / immunology ; Adenocarcinoma / diagnosis ; Adult ; Aged, 80 and over ; Biomarkers, Tumor / immunology Contributed Indexing: Keywords: Breast; Cancer; Immunofluorescence; Lung; Microenvironment; Pleural effusion Substance Nomenclature: 0 (B7-H1 Antigen) ; 0 (CD274 protein, human) ; 0 (Biomarkers, Tumor) Entry Date(s): Date Created: 20240322 Date Completed: 20240511 Latest Revision: 20240511 Update Code: 20240512

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