Einzeltreffer — DigiBib

Macrophages are important target cells for diverse viruses and thus represent a valuable system for studying virus biology. Isolation of primary human macrophages is done by culture of dissociated tissues or from differentiated blood monocytes, but these methods are both time consuming and result in low numbers of recovered macrophages. Here, we explore whether macrophages derived from human induced pluripotent stem cells (iPSCs)-which proliferate indefinitely and potentially provide unlimited starting material-could serve as a faithful model system for studying virus biology. Human iPSC-derived monocytes were differentiated into macrophages and then infected with HIV-1, dengue virus, or influenza virus as model human viruses. We show that iPSC-derived macrophages support the replication of these viruses with kinetics and phenotypes similar to human blood monocyte-derived macrophages. These iPSC-derived macrophages were virtually indistinguishable from human blood monocyte-derived macrophages based on surface marker expression (flow cytometry), transcriptomics (RNA sequencing), and chromatin accessibility profiling. iPSC lines were additionally generated from non-human primate (chimpanzee) fibroblasts. When challenged with dengue virus, human and chimpanzee iPSC-derived macrophages show differential susceptibility to infection, thus providing a valuable resource for studying the species-tropism of viruses. We also show that blood- and iPSC-derived macrophages both restrict influenza virus at a late stage of the virus lifecycle. Collectively, our results substantiate iPSC-derived macrophages as an alternative to blood monocyte-derived macrophages for the study of virus biology.

Importance: Macrophages have complex relationships with viruses: while macrophages aid in the removal of pathogenic viruses from the body, macrophages are also manipulated by some viruses to serve as vessels for viral replication, dissemination, and long-term persistence. Here, we show that iPSC-derived macrophages are an excellent model that can be exploited in virology.

Competing Interests: Q.Y., R.D.D., and S.L.S. are co-founders and/or consultants for Darwin Biosciences.

Titel:	Macrophages derived from human induced pluripotent stem cells (iPSCs) serve as a high-fidelity cellular model for investigating HIV-1, dengue, and influenza viruses.
Autor/in / Beteiligte Person:	Yang, Q ; Barbachano-Guerrero, A ; Fairchild, LM ; Rowland, TJ ; Dowell, RD ; Allen, MA ; Warren, CJ ; Sawyer, SL
Zeitschrift:	Journal of virology, Jg. 98 (2024-03-19), Heft 3, S. e0156323
Veröffentlichung:	Washington Dc : American Society For Microbiology ; <i>Original Publication</i>: Baltimore, American Society for Microbiology., 2024
Medientyp:	academicJournal
ISSN:	1098-5514 (electronic)
DOI:	10.1128/jvi.01563-23
Schlagwort:	Animals Humans Cell Differentiation genetics Pan troglodytes Fibroblasts cytology Monocytes cytology Virus Replication Flow Cytometry Gene Expression Profiling Chromatin Assembly and Disassembly Viral Tropism Biomarkers analysis Biomarkers metabolism HIV-1 growth & development HIV-1 physiology Induced Pluripotent Stem Cells cytology Macrophages cytology Macrophages metabolism Macrophages virology Orthomyxoviridae growth & development Orthomyxoviridae physiology Dengue Virus growth & development Dengue Virus physiology Models, Biological Virology methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Virol] 2024 Mar 19; Vol. 98 (3), pp. e0156323. <i>Date of Electronic Publication: </i>2024 Feb 07. MeSH Terms: HIV-1* / growth & development ; HIV-1* / physiology ; Induced Pluripotent Stem Cells* / cytology ; Macrophages* / cytology ; Macrophages* / metabolism ; Macrophages* / virology ; Orthomyxoviridae* / growth & development ; Orthomyxoviridae* / physiology ; Dengue Virus* / growth & development ; Dengue Virus* / physiology ; Models, Biological* ; Virology* / methods ; Animals ; Humans ; Cell Differentiation / genetics ; Pan troglodytes ; Fibroblasts / cytology ; Monocytes / cytology ; Virus Replication ; Flow Cytometry ; Gene Expression Profiling ; Chromatin Assembly and Disassembly ; Viral Tropism ; Biomarkers / analysis ; Biomarkers / metabolism References: Methods Mol Biol. 2021;2244:103-113. (PMID: 33555584) ; Int J Mol Sci. 2018 Jun 19;19(6):. (PMID: 29921749) ; Methods Mol Biol. 2018;1784:119-126. (PMID: 29761394) ; J Virol. 2015 Dec;89(24):12319-29. (PMID: 26423941) ; Crit Rev Immunol. 2012;32(6):463-88. (PMID: 23428224) ; Am J Trop Med Hyg. 2001 Nov;65(5):427-34. (PMID: 11716094) ; J Exp Med. 2019 Mar 4;216(3):482-500. (PMID: 30755454) ; J Virol. 2007 Jul;81(13):7111-23. (PMID: 17475660) ; J Gen Virol. 2017 Oct;98(10):2401-2412. (PMID: 28884667) ; PLoS Negl Trop Dis. 2007 Nov 21;1(2):e86. (PMID: 18060089) ; J Med Virol. 2000 Apr;60(4):425-31. (PMID: 10686026) ; Am J Trop Med Hyg. 1978 May;27(3):590-9. (PMID: 677372) ; J Infect Dis. 1999 Apr;179(4):755-62. (PMID: 10068569) ; J Med Virol. 1982;9(3):177-84. (PMID: 7097255) ; PLoS One. 2017 Oct 26;12(10):e0185056. (PMID: 29073143) ; Blood. 2013 Feb 28;121(9):e57-69. (PMID: 23293084) ; Immunol Lett. 1996 Nov;53(2-3):115-20. (PMID: 9024988) ; J Exp Med. 1977 Jul 1;146(1):218-29. (PMID: 195000) ; Eur J Immunol. 2014 Jul;44(7):2003-12. (PMID: 24687623) ; Nat Rev Immunol. 2011 Oct 10;11(11):762-74. (PMID: 21984070) ; Genes (Basel). 2020 May 06;11(5):. (PMID: 32384610) ; Int J Mol Sci. 2018 Sep 18;19(9):. (PMID: 30231586) ; Nat Protoc. 2022 Feb;17(2):513-539. (PMID: 35039668) ; Cells. 2020 May 29;9(6):. (PMID: 32485910) ; Curr Top Microbiol Immunol. 2017;407:65-82. (PMID: 28688086) ; Ann Neurol. 1988;23 Suppl:S58-61. (PMID: 3258140) ; OMICS. 2012 May;16(5):284-7. (PMID: 22455463) ; Front Immunol. 2019 Jun 26;10:1435. (PMID: 31297114) ; Genome Biol. 2014;15(12):550. (PMID: 25516281) ; Int J Mol Sci. 2020 Jul 07;21(13):. (PMID: 32645954) ; Am J Respir Crit Care Med. 2018 Aug 1;198(3):350-360. (PMID: 29652170) ; Biomolecules. 2021 May 06;11(5):. (PMID: 34066618) ; Exp Hematol. 1999 Feb;27(2):353-64. (PMID: 10029175) ; Nat Biotechnol. 2019 Aug;37(8):907-915. (PMID: 31375807) ; Nucleic Acids Res. 2013 May 1;41(10):e108. (PMID: 23558742) ; PLoS One. 2012;7(3):e29879. (PMID: 22396727) ; J Immunol. 2017 Feb 15;198(4):1616-1626. (PMID: 28062701) ; Nucleic Acids Res. 2015 Jul 1;43(W1):W39-49. (PMID: 25953851) ; Front Immunol. 2019 Jul 03;10:1462. (PMID: 31333642) ; J Virol. 2012 Dec;86(23):12561-70. (PMID: 22973027) ; Neuron. 2017 Apr 19;94(2):278-293.e9. (PMID: 28426964) ; Am J Trop Med Hyg. 1993 Mar;48(3):324-31. (PMID: 8470771) ; Annu Rev Physiol. 2017 Feb 10;79:541-566. (PMID: 27813830) ; Int Immunopharmacol. 2014 Nov;23(1):37-45. (PMID: 25130606) ; Stem Cell Reports. 2018 Aug 14;11(2):348-362. (PMID: 29983385) ; J Virol Methods. 2000 Oct;90(1):51-7. (PMID: 11011080) ; J Clin Invest. 2009 Sep;119(9):2818-29. (PMID: 19726877) ; J Immunol. 2005 Jun 15;174(12):8163-72. (PMID: 15944325) ; Am J Pathol. 2014 Jan;184(1):171-83. (PMID: 24200852) ; Life Sci. 2021 Mar 15;269:119010. (PMID: 33454368) ; Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308) ; J Virol. 1997 Feb;71(2):1657-61. (PMID: 8995695) ; J Leukoc Biol. 2010 Apr;87(4):599-608. (PMID: 20042468) ; Free Radic Biol Med. 2013 Jan;54:1-16. (PMID: 23124025) ; J Leukoc Biol. 2019 Aug;106(2):345-358. (PMID: 30576000) ; Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) ; J Virol. 1999 Apr;73(4):2650-7. (PMID: 10074110) ; J Virol. 2002 Oct;76(19):9877-87. (PMID: 12208965) ; Neuron. 2019 Oct 23;104(2):239-255.e12. (PMID: 31422865) ; Stem Cell Reports. 2016 Jun 14;6(6):873-884. (PMID: 27161365) ; J Virol. 1992 Apr;66(4):2232-9. (PMID: 1548759) ; Stem Cell Res. 2020 Jul;46:101851. (PMID: 32450543) ; J Immunol. 2000 Jun 15;164(12):6166-73. (PMID: 10843666) ; Curr Protoc Stem Cell Biol. 2020 Mar;52(1):e108. (PMID: 32159928) ; Arch Virol. 1990;110(1-2):91-101. (PMID: 2178591) ; Nat Rev Genet. 2011 Apr;12(4):253-65. (PMID: 21415849) ; Biomaterials. 2019 Feb;192:377-391. (PMID: 30497023) ; J Immunol. 1998 Dec 1;161(11):6338-46. (PMID: 9834124) ; J Virol. 2008 May;82(9):4265-74. (PMID: 18287245) ; Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408) ; Blood. 1979 Aug;54(2):485-500. (PMID: 454850) ; Exp Hematol. 2019 Mar;71:13-23. (PMID: 30611869) ; J Virol. 2011 Jul;85(14):6844-55. (PMID: 21543489) ; Bio Protoc. 2020 Dec 20;10(24):e3854. (PMID: 33659501) ; Infect Immun. 1977 Oct;18(1):151-6. (PMID: 409682) ; Nat Commun. 2018 Nov 30;9(1):5088. (PMID: 30504915) ; Front Microbiol. 2021 Apr 07;12:646447. (PMID: 33897659) ; Immunity. 2017 Jul 18;47(1):183-198.e6. (PMID: 28723550) ; J Virol. 2010 Aug;84(15):7569-80. (PMID: 20504924) ; Nat Methods. 2017 Oct;14(10):959-962. (PMID: 28846090) ; Stem Cell Reports. 2019 Jun 11;12(6):1282-1297. (PMID: 31189095) ; Nat Rev Immunol. 2018 Nov;18(11):716-725. (PMID: 30140052) ; J Virol. 2004 May;78(9):4665-74. (PMID: 15078949) ; Stem Cell Reports. 2015 Feb 10;4(2):282-96. (PMID: 25680479) ; Mol Immunol. 2013 Jun;54(2):157-63. (PMID: 23287596) ; Front Immunol. 2014 Oct 17;5:514. (PMID: 25368618) ; Nat Commun. 2018 Feb 21;9(1):745. (PMID: 29467427) ; Cell Rep. 2021 Nov 2;37(5):109955. (PMID: 34731634) ; Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11531-6. (PMID: 20534532) ; STAR Protoc. 2023 May 18;4(2):102291. (PMID: 37209094) ; Annu Rev Immunol. 2014;32:513-45. (PMID: 24555472) ; PLoS Pathog. 2014 Apr 03;10(4):e1004053. (PMID: 24699679) ; Science. 2007 Dec 21;318(5858):1917-20. (PMID: 18029452) ; Front Immunol. 2019 May 24;10:1084. (PMID: 31178859) ; Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10736-41. (PMID: 12149435) Grant Information: DP1 DA046108 United States DA NIDA NIH HHS; DP1 AI175471 United States AI NIAID NIH HHS; S10 OD021601 United States OD NIH HHS; R01 AI137011 United States AI NIAID NIH HHS; K99 AI151256 United States AI NIAID NIH HHS; R01 HL156475 United States HL NHLBI NIH HHS; F32 GM125442 United States GM NIGMS NIH HHS; R00 AI151256 United States AI NIAID NIH HHS; R01 OD034046 United States OD NIH HHS Contributed Indexing: Keywords: ATACseq; RNAseq; dengue virus; human immunodeficiency virus; iPSC-derived macrophages; induced pluripotent stem cells; influenza virus; macrophages Substance Nomenclature: 0 (Biomarkers) Entry Date(s): Date Created: 20240207 Date Completed: 20240320 Latest Revision: 20240621 Update Code: 20240621 PubMed Central ID: PMC10949493

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Macrophages derived from human induced pluripotent stem cells (iPSCs) serve as a high-fidelity cellular model for investigating HIV-1, dengue, and influenza viruses.