Einzeltreffer — DigiBib

Omega-3 polyunsaturated fatty acids (n-3 PUFA), such as the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are reported to beneficially affect the intestinal immunity. The biological pathways modulated by n-3 PUFA during an infection, at the level of intestinal epithelial barrier remain elusive. To address this gap, we investigated the proteomic changes induced by n-3 PUFA in porcine enterocyte cell line (IPEC-J2), in the presence and absence of lipopolysaccharide (LPS) stress conditions using shotgun proteomics analysis integrated with RNA-sequencing technology. A total of 33, 85, and 88 differentially abundant proteins (DAPs) were identified in cells exposed to n-3 PUFA (DHA:EPA), LPS, and n-3 PUFA treatment followed by LPS stimulation, respectively. Functional annotation and pathway analysis of DAPs revealed the modulation of central carbon metabolism, including the glycolysis/gluconeogenesis, pentose phosphate pathway, and oxidative phosphorylation processes. Specifically, LPS caused metabolic dysregulation in enterocytes, which was abated upon prior treatment with n-3 PUFA. Besides, n-3 PUFA supplementation facilitated enterocyte development and lipid homeostasis. Altogether, this work for the first time comprehensively described the biological pathways regulated by n-3 PUFA in enterocytes, particularly during endotoxin-stimulated metabolic dysregulation. Additionally, this study may provide nutritional biomarkers in monitoring the intestinal health of human and animals on n-3 PUFA-based diets.

Titel:	Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes.
Autor/in / Beteiligte Person:	Sundaram, TS ; Addis, MF ; Giromini, C ; Rebucci, R ; Pisanu, S ; Pagnozzi, D ; Baldi, A
Zeitschrift:	Scientific reports, Jg. 13 (2023-12-07), Heft 1, S. 21595
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2023
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-023-48018-3
Schlagwort:	Humans Animals Swine Enterocytes metabolism Endotoxins Lipopolysaccharides pharmacology Proteomics Eicosapentaenoic Acid metabolism Docosahexaenoic Acids metabolism Fatty Acids metabolism Fatty Acids, Omega-3 pharmacology Fatty Acids, Omega-3 metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Sci Rep] 2023 Dec 07; Vol. 13 (1), pp. 21595. <i>Date of Electronic Publication: </i>2023 Dec 07. MeSH Terms: Fatty Acids, Omega-3* / pharmacology ; Fatty Acids, Omega-3* / metabolism ; Humans ; Animals ; Swine ; Enterocytes / metabolism ; Endotoxins ; Lipopolysaccharides / pharmacology ; Proteomics ; Eicosapentaenoic Acid / metabolism ; Docosahexaenoic Acids / metabolism ; Fatty Acids / metabolism References: Cell Mol Life Sci. 2021 Feb;78(4):1233-1261. (PMID: 33057840) ; Proc Nutr Soc. 2012 May;71(2):284-9. (PMID: 22369781) ; Vet Immunol Immunopathol. 2009 Oct 15;131(3-4):278-84. (PMID: 19446887) ; Curr Opin Pharmacol. 2006 Aug;6(4):414-20. (PMID: 16750421) ; Nat Immunol. 2001 Jul;2(7):612-9. (PMID: 11429545) ; Cancer Res. 2008 Oct 15;68(20):8201-9. (PMID: 18922891) ; Cold Spring Harb Symp Quant Biol. 2011;76:285-9. (PMID: 22096030) ; Nutrients. 2010 Mar;2(3):355-374. (PMID: 22254027) ; J Biol Chem. 2005 Oct 7;280(40):33766-74. (PMID: 16081425) ; Front Immunol. 2020 Feb 20;11:202. (PMID: 32153568) ; FEBS J. 2020 Aug;287(16):3350-3369. (PMID: 32255251) ; Vet Res. 2020 Mar 31;51(1):49. (PMID: 32234079) ; J Biol Chem. 2009 Dec 11;284(50):34538-44. (PMID: 19850935) ; J Am Soc Mass Spectrom. 2016 Nov;27(11):1719-1727. (PMID: 27572102) ; Trends Food Sci Technol. 2011 Nov;22:S11-S20. (PMID: 32336880) ; Lipids Health Dis. 2013 Jul 13;12:101. (PMID: 23849180) ; Prog Lipid Res. 1995;34(2):139-50. (PMID: 7480063) ; Nat Commun. 2022 Jan 10;13(1):165. (PMID: 35013197) ; Vet Res. 2018 Dec 4;49(1):117. (PMID: 30514405) ; Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Oct;1862(10 Pt B):1221-1232. (PMID: 28754637) ; Infect Immun. 2011 Apr;79(4):1759-69. (PMID: 21321075) ; Mol Biol Cell. 2010 Sep 1;21(17):2996-3006. (PMID: 20631255) ; Scand J Immunol. 2018 Nov;88(5):e12713. (PMID: 30176060) ; iScience. 2020 May 22;23(5):101116. (PMID: 32417402) ; Proc Natl Acad Sci U S A. 1971 Dec;68(12):2982-5. (PMID: 5289242) ; Eur J Clin Nutr. 2002 Aug;56 Suppl 3:S14-9. (PMID: 12142955) ; FEBS J. 2018 Jun;285(12):2146-2160. (PMID: 29603622) ; Prostaglandins Leukot Essent Fatty Acids. 2017 Jan;116:27-31. (PMID: 28088291) ; Animals (Basel). 2020 May 31;10(6):. (PMID: 32486441) ; PLoS Pathog. 2021 Sep 23;17(9):e1009943. (PMID: 34555129) ; Front Physiol. 2014 Jul 31;5:282. (PMID: 25132820) ; Sci Rep. 2019 Nov 1;9(1):15850. (PMID: 31676851) ; J Anim Sci Biotechnol. 2022 Apr 11;13(1):40. (PMID: 35399093) ; Prog Mol Biol Transl Sci. 2011;100:369-88. (PMID: 21377631) ; BMC Biol. 2013 Jan 23;11:3. (PMID: 23342984) ; Nat Methods. 2007 Nov;4(11):923-5. (PMID: 17952086) ; Nat Immunol. 2005 Dec;6(12):1191-7. (PMID: 16369558) ; Biomol Ther (Seoul). 2018 Jan 1;26(1):29-38. (PMID: 29212304) ; Anticancer Agents Med Chem. 2008 Jan;8(1):2-21. (PMID: 18220502) ; Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Jan;1866(1):158840. (PMID: 33049404) ; Viruses. 2018 Sep 24;10(10):. (PMID: 30249998) ; Br J Clin Pharmacol. 2013 Mar;75(3):645-62. (PMID: 22765297) ; Infect Immun. 2011 Sep;79(9):3833-45. (PMID: 21690237) ; Infect Immun. 2013 Sep;81(9):3182-97. (PMID: 23774600) ; Braz J Med Biol Res. 1998 Apr;31(4):467-90. (PMID: 9698798) ; Sci Rep. 2020 Sep 14;10(1):15021. (PMID: 32929120) ; Curr Nutr Rep. 2014 Jun;3(2):139-148. (PMID: 24977108) ; J Nutr. 2005 Apr;135(4):687-94. (PMID: 15795419) ; J Biol Chem. 2018 Apr 13;293(15):5509-5521. (PMID: 29463677) ; Front Endocrinol (Lausanne). 2020 Jun 09;11:365. (PMID: 32582032) ; Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243) ; Sci Rep. 2019 Dec 10;9(1):18763. (PMID: 31822804) ; Biochim Biophys Acta. 2015 Apr;1851(4):469-84. (PMID: 25149823) ; Nat Rev Immunol. 2008 May;8(5):349-61. (PMID: 18437155) ; Oncol Lett. 2012 Dec;4(6):1151-1157. (PMID: 23226794) Substance Nomenclature: 0 (Fatty Acids, Omega-3) ; 0 (Endotoxins) ; 0 (Lipopolysaccharides) ; AAN7QOV9EA (Eicosapentaenoic Acid) ; 25167-62-8 (Docosahexaenoic Acids) ; 0 (Fatty Acids) Entry Date(s): Date Created: 20231207 Date Completed: 20231216 Latest Revision: 20231222 Update Code: 20231222 PubMed Central ID: PMC10703801

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes.