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Objective: Effects of tea catechin epigallocatechin-3-gallate (EGCG) against biofilm formation by Streptococcus mutans and probiotic Lactobacillus casei in Yakult ® (LcY) were examined.

Design: Biofilms were formed by S. mutans alone (Sm) and co-culture of S. mutans and LcY (Sm + LcY) in the absence or presence of EGCG. The biomass of biofilms, which were sonicated or not, was measured by the crystal violet assay. Biofilm morphology was observed by scanning electron microscopy. Bacterial viability and extracellular polysaccharides were determined by SYTO9/propidium iodide and dextran-conjugated fluorescein staining, respectively, and confocal microscopy. Gene expression of glucosyltransferase was determined by quantitative polymerase chain reaction.

Results: While 250 μg/ml EGCG significantly decreased the biomass and acid production of Sm biofilms, 500 μg/ml EGCG was required to inhibit Sm + LcY biofilm formation and acid production. EGCG decreased the amount of live bacteria present in both Sm and Sm + LcY biofilms. The level of dead bacteria in Sm + LcY biofilms was higher than in Sm biofilms when formed in the presence of 250 μg/ml EGCG. EGCG decreased levels of extracellular polysaccharides in Sm and Sm + LcY biofilms. The extent of biofilm removal by sonication was not different between Sm and Sm+LcY biofilms formed in the absence or presence of 62.5 or 125 μg/ml EGCG. The level of Sm gtfB and gtfD expression in Sm + LcY biofilms was higher than those in the Sm biofilms when formed in the presence of EGCG at 250 μg/ml.

Conclusion: The results indicated that LcY might interfere the inhibitory effects of EGCG against biofilm formation by S. mutans.

Titel:	Inhibitory effects of tea catechin epigallocatechin-3-gallate against biofilms formed from Streptococcus mutans and a probiotic lactobacillus strain.
Autor/in / Beteiligte Person:	Wu, CY ; Su, TY ; Wang, MY ; Yang, SF ; Mar, K ; Hung, SL
Zeitschrift:	Archives of oral biology, Jg. 94 (2018-10-01), S. 69-77
Veröffentlichung:	Oxford : Pergamon Press ; <i>Original Publication</i>: Oxford, New York [etc.] Pergamon Press., 2018
Medientyp:	academicJournal
ISSN:	1879-1506 (electronic)
DOI:	10.1016/j.archoralbio.2018.06.019
Schlagwort:	Bacterial Proteins metabolism Biofilms growth & development Biomass Dose-Response Relationship, Drug Gene Expression Regulation, Bacterial drug effects Gene Expression Regulation, Bacterial genetics Glucosyltransferases metabolism Lacticaseibacillus casei genetics Lacticaseibacillus casei metabolism Microbial Sensitivity Tests Microbial Viability drug effects Polysaccharides metabolism Streptococcus mutans genetics Streptococcus mutans metabolism Anti-Bacterial Agents pharmacology Biofilms drug effects Catechin analogs & derivatives Catechin antagonists & inhibitors Lacticaseibacillus casei drug effects Probiotics Streptococcus mutans drug effects Tea chemistry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Arch Oral Biol] 2018 Oct; Vol. 94, pp. 69-77. <i>Date of Electronic Publication: </i>2018 Jun 26. MeSH Terms: Probiotics* ; Anti-Bacterial Agents / pharmacology ; Biofilms / drug effects ; Catechin / analogs & derivatives ; Catechin / antagonists & inhibitors ; Lacticaseibacillus casei / drug effects ; Streptococcus mutans / drug effects ; Tea / *chemistry ; Bacterial Proteins / metabolism ; Biofilms / growth & development ; Biomass ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Bacterial / drug effects ; Gene Expression Regulation, Bacterial / genetics ; Glucosyltransferases / metabolism ; Lacticaseibacillus casei / genetics ; Lacticaseibacillus casei / metabolism ; Microbial Sensitivity Tests ; Microbial Viability / drug effects ; Polysaccharides / metabolism ; Streptococcus mutans / genetics ; Streptococcus mutans / metabolism Contributed Indexing: Keywords: Biofilms; Epigallocatechin-3-gallate; Lactobacillus casei; Probiotic; Streptococcus mutans Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (Bacterial Proteins) ; 0 (Polysaccharides) ; 0 (Tea) ; 8R1V1STN48 (Catechin) ; BQM438CTEL (epigallocatechin gallate) ; EC 2.4.1.- (1,3-alpha-D-glucan synthase) ; EC 2.4.1.- (Glucosyltransferases) ; EC 2.4.1.- (glucosyltransferase D) Entry Date(s): Date Created: 20180707 Date Completed: 20190211 Latest Revision: 20221207 Update Code: 20231215

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Inhibitory effects of tea catechin epigallocatechin-3-gallate against biofilms formed from Streptococcus mutans and a probiotic lactobacillus strain.