Epigallocatechin-3-gallate (EGCG) Suppresses the Trafficking of Lymphocytes to Epidermal Melanocytes via Inhibition of JAK2: Its Implication for Vitiligo Treatment.
In: Biological & pharmaceutical bulletin, Jg. 38 (2015), Heft 11, S. 1700-6
academicJournal
Zugriff:
Vitiligo is an inflammatory skin disorder in which activated T cells play an important role in its onset and progression. Epigallocatechin-3-gallate (EGCG), the major chemical constituent of green tea, exhibits remarkable anti-oxidative and anti-inflammatory properties. EGCG administration has been confirmed to decrease the risk of vitiligo; however, the underlying mechanism is undetermined. In this study, we proved that EGCG directly inhibited the kinase activity of Janus kinase 2 (JAK2). In primary cultured human melanocytes, EGCG pre-treatment attenuated interferon (IFN)-γ-induced phosphorylation of JAK2 and its downstream signal transducer and activator of transcription (STAT)1 and STAT3 in a dose-dependent manner. We further examined the chemoattractant expression in melanocytes and demonstrated that EGCG significantly inhibited IFN-γ-induced expression of intracellular adhesion molecule (ICAM)-1, CXCL10, and monocyte chemotactic protein (MCP)-1 in human melanocytes. In addition, EGCG reduced the protein levels of the corresponding receptors including CD11a, CXCR3, and CCR2 in human T lymphocytes. As a consequence, adhesion of human T cells to melanocytes induced by IFN-γ was effectively suppressed by EGCG. Taken together, our results provided new evidence for the effectiveness of EGCG in vitiligo treatment and supported JAK2 as a molecular target for vitiligo medicine development.
Titel: |
Epigallocatechin-3-gallate (EGCG) Suppresses the Trafficking of Lymphocytes to Epidermal Melanocytes via Inhibition of JAK2: Its Implication for Vitiligo Treatment.
|
---|---|
Autor/in / Beteiligte Person: | Ning, W ; Wang, S ; Dong, X ; Liu, D ; Fu, L ; Jin, R ; Xu, A |
Zeitschrift: | Biological & pharmaceutical bulletin, Jg. 38 (2015), Heft 11, S. 1700-6 |
Veröffentlichung: | Tokyo : Pharmaceutical Society of Japan, c1993-, 2015 |
Medientyp: | academicJournal |
ISSN: | 1347-5215 (electronic) |
DOI: | 10.1248/bpb.b15-00331 |
Schlagwort: |
|
Sonstiges: |
|