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NKAP is a highly conserved protein with roles in transcriptional repression, T-cell development, maturation and acquisition of functional competency and maintenance and survival of adult hematopoietic stem cells. Here we report the novel role of NKAP in splicing. With NKAP-specific antibodies we found that NKAP localizes to nuclear speckles. NKAP has an RS motif at the N-terminus followed by a highly basic domain and a DUF 926 domain at the C-terminal region. Deletion analysis showed that the basic domain is important for speckle localization. In pull-down experiments, we identified RNA-binding proteins, RNA helicases and splicing factors as interaction partners of NKAP, among them FUS/TLS. The FUS/TLS-NKAP interaction takes place through the RS domain of NKAP and the RGG1 and RGG3 domains of FUS/TLS. We analyzed the ability of NKAP to interact with RNA using in vitro splicing assays and found that NKAP bound both spliced messenger RNA (mRNA) and unspliced pre-mRNA. Genome-wide analysis using crosslinking and immunoprecipitation-seq revealed NKAP association with U1, U4 and U5 small nuclear RNA, and we also demonstrated that knockdown of NKAP led to an increase in pre-mRNA percentage. Our results reveal NKAP as nuclear speckle protein with roles in RNA splicing and processing.

Titel:	NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.
Autor/in / Beteiligte Person:	Burgute, BD ; Peche, VS ; Steckelberg, AL ; Glöckner, G ; Gaßen, B ; Gehring, NH ; Noegel, AA
Zeitschrift:	Nucleic acids research, Jg. 42 (2014-03-01), Heft 5, S. 3177-93
Veröffentlichung:	1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 2014
Medientyp:	academicJournal
ISSN:	1362-4962 (electronic)
DOI:	10.1093/nar/gkt1311
Schlagwort:	Animals Cell Nucleus HEK293 Cells HeLa Cells Humans Mice Nuclear Proteins analysis Protein Structure, Tertiary RNA Helicases metabolism RNA Splicing RNA, Small Nuclear metabolism RNA-Binding Protein FUS metabolism RNA-Binding Proteins metabolism Repressor Proteins analysis Repressor Proteins chemistry RNA metabolism Repressor Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Nucleic Acids Res] 2014 Mar; Vol. 42 (5), pp. 3177-93. <i>Date of Electronic Publication: </i>2013 Dec 17. MeSH Terms: RNA / metabolism ; Repressor Proteins / metabolism ; Animals ; Cell Nucleus ; HEK293 Cells ; HeLa Cells ; Humans ; Mice ; Nuclear Proteins / analysis ; Protein Structure, Tertiary ; RNA Helicases / metabolism ; RNA Splicing ; RNA, Small Nuclear / metabolism ; RNA-Binding Protein FUS / metabolism ; RNA-Binding Proteins / metabolism ; Repressor Proteins / analysis ; Repressor Proteins / chemistry References: Mol Biol Cell. 2012 May;23(9):1765-82. (PMID: 22419818) ; RNA. 2010 Dec;16(12):2384-403. (PMID: 20980672) ; Cold Spring Harb Perspect Biol. 2011 Feb 01;3(2):. (PMID: 20926517) ; Nucleic Acids Res. 2012 Mar;40(6):2639-52. (PMID: 22110043) ; Sci Rep. 2012;2:529. (PMID: 22829983) ; Mol Biol Cell. 2010 Feb 15;21(4):650-63. 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(PMID: 9774382) Substance Nomenclature: 0 (NKAP protein, mouse) ; 0 (Nuclear Proteins) ; 0 (RNA, Small Nuclear) ; 0 (RNA-Binding Protein FUS) ; 0 (RNA-Binding Proteins) ; 0 (Repressor Proteins) ; 63231-63-0 (RNA) ; EC 3.6.4.13 (RNA Helicases) Entry Date(s): Date Created: 20131220 Date Completed: 20140519 Latest Revision: 20220330 Update Code: 20231215 PubMed Central ID: PMC3950704

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NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.