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We use the temporal asymmetry of the cross-correlation function to determine the temporal ordering of spatially localized cellular events in live-cell multichannel fluorescence imaging. The analysis is well suited to noisy, stochastic systems where the temporal order may not be apparent in the raw data. The approach is applicable to any biochemical reaction not in chemical equilibrium, including protein complex assembly, sequential enzymatic processes, gene regulation, and other cellular signaling events. As an automated quantitative measure, this approach allows the data to be readily interpreted statistically with minimal subjective biases. We first test the technique using simulations of simple biophysical models with a definite temporal ordering. We then demonstrate the approach by extracting the temporal ordering of three proteins-actin, sorting nexin 9, and clathrin-in the endocytic pathway.

Titel:	Event ordering in live-cell imaging determined from temporal cross-correlation asymmetry.
Autor/in / Beteiligte Person:	Sisan, DR ; Yarar, D ; Waterman, CM ; Urbach, JS
Zeitschrift:	Biophysical journal, Jg. 98 (2010-06-02), Heft 11, S. 2432-41
Veröffentlichung:	Cambridge, MA : Cell Press ; <i>Original Publication</i>: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society., 2010
Medientyp:	academicJournal
ISSN:	1542-0086 (electronic)
DOI:	10.1016/j.bpj.2010.02.041
Schlagwort:	Actins chemistry Algorithms Animals Carrier Proteins chemistry Cell Line Chlorocebus aethiops Clathrin chemistry Monte Carlo Method Signal Processing, Computer-Assisted Signal Transduction Sorting Nexins Stochastic Processes Time Factors Vesicular Transport Proteins chemistry Video Recording methods Cell Physiological Phenomena Computer Simulation Microscopy, Fluorescence methods Models, Biological Proteins chemistry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Biophys J] 2010 Jun 02; Vol. 98 (11), pp. 2432-41. MeSH Terms: Cell Physiological Phenomena* ; Computer Simulation* ; Models, Biological* ; Microscopy, Fluorescence / methods ; Proteins / chemistry ; Actins / chemistry ; Algorithms ; Animals ; Carrier Proteins / chemistry ; Cell Line ; Chlorocebus aethiops ; Clathrin / chemistry ; Monte Carlo Method ; Signal Processing, Computer-Assisted ; Signal Transduction ; Sorting Nexins ; Stochastic Processes ; Time Factors ; Vesicular Transport Proteins / chemistry ; Video Recording / methods References: Biophys J. 2002 Aug;83(2):1184-93. (PMID: 12124298) ; Nat Methods. 2006 Feb;3(2):83-9. (PMID: 16432516) ; Science. 2005 Apr 22;308(5721):523-9. (PMID: 15845847) ; Science. 2006 Apr 14;312(5771):217-24. (PMID: 16614209) ; Traffic. 2001 Nov;2(11):789-96. (PMID: 11733045) ; Biophys J. 2008 Jun;94(12):4996-5008. (PMID: 18326662) ; Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2828-33. (PMID: 14970342) ; Chemphyschem. 2005 May;6(5):984-90. (PMID: 15884086) ; Dev Cell. 2007 Jul;13(1):43-56. (PMID: 17609109) ; Biophys J. 2006 Jan 15;90(2):628-39. (PMID: 16258048) ; Biophys J. 1986 Jul;50(1):171-9. (PMID: 3730501) ; Circ Res. 2000 Sep 29;87(7):526-8. (PMID: 11009552) ; Mol Biol Cell. 2005 Feb;16(2):964-75. (PMID: 15601897) ; Biophys J. 1993 Sep;65(3):1135-46. (PMID: 8241393) ; Biophys J. 2009 Jan;96(2):707-16. (PMID: 19167315) ; Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1416-20. (PMID: 9465029) ; Free Radic Biol Med. 2000 May 15;28(10):1456-62. (PMID: 10927169) ; Nat Rev Cancer. 2006 Feb;6(2):146-55. (PMID: 16491074) ; Biophys J. 1997 Apr;72(4):1878-86. (PMID: 9083691) Substance Nomenclature: 0 (Actins) ; 0 (Carrier Proteins) ; 0 (Clathrin) ; 0 (Proteins) ; 0 (Sorting Nexins) ; 0 (Vesicular Transport Proteins) Entry Date(s): Date Created: 20100602 Date Completed: 20100902 Latest Revision: 20211020 Update Code: 20240513 PubMed Central ID: PMC2877330

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Event ordering in live-cell imaging determined from temporal cross-correlation asymmetry.