YAP/Yorkie in the germline modulates the age-related decline of germline stem cells and niche cells.
In: PLoS ONE, Jg. 14 (2019-04-03), Heft 4, S. 1-16
academicJournal
Zugriff:
The properties and behaviour of stem cells rely heavily on signaling from the local microenvironment. At the apical end of Drosophila testis, self-renewal and differentiation of germline stem cells (GSCs) are tightly controlled by distinct somatic cells that comprise a specialised stem cell niche known as the hub. The hub maintains GSC homeostasis through adhesion and cell signaling. The Salvador/Warts/Hippo (SWH) pathway, which suppresses the transcriptional co-activator YAP/Yki via a kinase cascade, is a known regulator of stem cell proliferation and differentiation. Here, we show that increasing YAP/Yki expression in the germline, as well as reducing Warts levels, blocks the decrease of GSC numbers observed in aging flies, with only a small increase on their proliferation. An increased expression of YAP/Yki in the germline or a reduction in Warts levels also stymies an age-related reduction in hub cell number, suggesting a bilateral relationship between GSCs and the hub. Conversely, RNAi-based knockdown of YAP/Yki in the germline leads to a significant drop in hub cell number, further suggesting the existence of such a SC-to-niche relationship. All together, our data implicate the SWH pathway in Drosophila GSC maintenance and raise questions about its role in stem cell homeostasis in aging organisms. [ABSTRACT FROM AUTHOR]
Titel: |
YAP/Yorkie in the germline modulates the age-related decline of germline stem cells and niche cells.
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Autor/in / Beteiligte Person: | Francis, Deepthy ; Chanana, Bhavna ; Fernandez, Beatriz ; Gordon, Benjamin ; Mak, Tiffany ; Palacios, Isabel M. |
Zeitschrift: | PLoS ONE, Jg. 14 (2019-04-03), Heft 4, S. 1-16 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0213327 |
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